‘The next is nanopores, that's our oldest line of investigation and that has now resulted in DNA sequencers determined by nanopores. We pull specific DNA strands by way of a nanopore and may then study off the nucleotide sequence. Really just lately, we even managed to go through out just one protein for the first time using This system. But this line of exploration is not simply about establishing resources, but additionally about real Organic inquiries.
Reconstitution of ultrawide DNA origami pores in liposomes for transmembrane transportation of macromolecules
‘It had been just a matter of buying up a reserve on mobile biology and starting to read. I'd come from a world of extremely-large vacuums, cryostats and quantum phenomena, and now I was Doing the job at area temperature with molecular biology kits.
Non-equilibrium folding of individual DNA molecules recaptured up to 1000 times within a sound state nanopore
This yielded a breakthrough. In only a few months, we ended up in a position to evaluate transport via someone single-walled carbon nanotube. Based upon low-temperature experiments by an excellent PhD student in my team, Sander Tans (now a valued colleague and professor at Delft College of Engineering using an Lively analysis lab at AMOLF in Amsterdam), we confirmed that carbon nanotubes were real quantum wires with pretty extended (micrometres) Digital coherence lengths — confirming theoretical predictions of metallic behaviour.
Electrical transport in monolayers of phthalocyanine molecular wires and AFM imaging of an individual wire bridging two electrodes
‘To start with we checked out biological techniques that has a Actual physical see and acted on The idea with the tools we knew. It took a several years in advance of we started Functioning additional on the massive Organic issues. I then started to get in touch with it nanobiology, a time period that did not exist at enough time.
Nanopores symbolize a chic and flexible Instrument to measure single molecules. We research novel detection modes with solid-point out nanopores and many tips on how to evaluate proteins with nanopores. Initiatives encompass the next:
‘You shouldn't just contemplate the issues of tomorrow, but will also evaluate the issues with the working day soon after tomorrow’
Very great, but that wasn’t definitely exactly where my fascination lay. I needed to pioneer and I had the feeling which was now not possible, since the most appealing essential insights had presently been uncovered. But my subject had formulated a lot of thrilling new applications to measure particular person molecules. I made a decision which i desired to use this to biology, to take a look at it from the Actual physical standpoint.’
Testing pseudotopological and nontopological styles for SMC-pushed DNA loop extrusion versus roadblock-traversal experiments
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One example is, we consider the nucleopore complex, a gateway concerning the cell nucleus and the cytoplasm. This is a really difficult system and as a consequence tough to study inside of a cell, so we use biomimetics. We reconstruct holes and then insert particular person factors of the nucleopore complex to review transport by it at the single-molecule stage.’
Dekker heeft fundamentele ontdekkingen gedaan op het https://www.ceesdekker.net gebied van koolstof nanobuisjes. Mede vanwege deze werkzaamheden is hij al jaren de meest geciteerde fysicus in Nederland. Tegenwoordig onderzoekt hij achieved enkel-molecuultechnieken de biofysica van moleculen in de biologische cel. Dekker is naast zijn wetenschappelijk werk ook bekend vanwege zijn belangstelling voor de relatie tussen geloof en wetenschap en de discussie rond intelligent design and style in 2005. (nl)